The ranking capacity between cultivars appeared to be very similar using either the VI or the PROSPECT based methods. 2013), the regional climate is Cfb: C indicates temperate climate, f is humid, without a dry season with monthly average rainfall no less than 60 mm, and b indicates average air temperature below 22 °C in the hottest month. Reflectance indices associated with physiological changes in nitrogen- and water-limited sunflower leaves. 2008;112(6):3030–43. 6a, b). The cost function $$J\left( V \right)$$ computed the distance between the PROSPECT simulated reflectance spectrum and the actual measurements over the 18 acquisitions performed on each date, cultivar and modality: The original 300–2500 nm spectral range of the ASD spectroradiometer was restricted to the 400–2200 nm domain because (1) the PROSPECT model was calibrated only for wavelengths higher than 400 nm and (2) the signal was dominated by noise for wavelengths longer than 2200 nm. Remote Sens Environ. PROSPECT simulates the directional hemispherical reflectance ($$R_{leaf}$$) and transmittance ($$T_{leaf}$$) of the leaf from the knowledge of the chlorophyll, carotenoid, water and dry matter contents, as well as brown pigments and the mesophyll structure parameter, $$N$$ [41, 46]. 2017). Key anatomical structures in relation to their mode of interaction with light. Plant reflectance is governed by leaf surface properties and internal structure, as the total chlorophyllian pigment content, $$C_{abc}$$, was therefore used in this study. It is not intended to provide medical or other professional advice. 1990;34:75–91. Tahara M, Carver BF, Johnson RC, Smith EL. Metrologia. The separation of anthocyanin from chlorophyllian pigments in PDb further decreased the RMSE between 500 to 600 nm where anthocyanin absorbs light. All the 36 modalities were sampled in April, while only 26 of them were collected in June. Saunderson J. 1862;11:545–56. Have any problems using the site? At 670 nm (wavelength associated with chlorophyll a), leaf reflectance increased 24% and 158% for initial and final GTD symptoms, respectively (Fig. HyperART: non-invasive quantification of leaf traits using hyperspectral absorption-reflectance-transmittance imaging. 2003;160(3):271–82. Results (Table 5) showed that, after bias correction, the performances of $$C_{\text{abc}}$$ estimation were good for all the versions of PROSPECT and similar to the ones of Dx4. Field Crops Res. Furthermore, new genotypes grown under given environmental conditions may have characteristics not well represented in the VI-relationship training database, making the biochemical content estimation uncertain. (3): where $$S_{ref\_bef} \left( \lambda \right)$$ and $$S_{ref\_aft} \left( \lambda \right)$$ are the spectra of the secondary Teflon reference completed before and after the series of the 18 leaf spectrophotometer measurements. Plant Methods 13:47, Kokaly RF, Skidmore AK (2015) Plant phenolics and absorption features in vegetation reflectance spectra near 1,66 μm. Leaf and canopy reflectance can be used to diagnose plant status. plant pathol. Masi et al. Estimating olive leaf nitrogen concentration using visible and near-infrared spectral reflectance. In this study, we showed that spectral response of asymptomatic grapevine leaves can be distinguished from symptomatic to two important diseases associated with vineyard decline. SLOP: a revised version of the stochastic model for leaf optical properties. The absolute $$DHRF_{ref}$$ of the secondary Teflon white reference was calibrated against a spectralon primary reference panel [52]. 1994;25(3–4):171–81. 2011). Meta-Analysis of the Detection of Plant Pigment Concentrations Using Hyperspectral Remotely Sensed Data. Oecologia. Plant Disease 98:1172–1185, Naidu RA, Maree HJ, Burger JT (2015) Grapevine leafroll disease and associated viruses: a unique pathosystem. 2011;16(12):635–44. 1995;53:199–211. Note that $$C_{bp}$$ was not measured since polyphenols are difficult to extract. PCA was performed by the Chemostat software (Helfer et al. Green and red points correspond to measurements achieved at two nodes (April) and grain filling (June) stages respectively. LEAFMOD: a new within-leaf radiative transfer model. Fluorescence imaging as a diagnostic tool for plant stress. Three initial guesses (Table 3) were used to avoid the algorithm to be trapped in a local minimum. Water content was estimated with a smaller bias, in relation to the more even distribution of the water in the leaf volume. Scatter plots between estimated anthocyanins and measured carotenoid from PROSPECT PDb (PROSPECT D considering the brown pigment content). 5f). The objective of this study was to evaluate the performances of the several versions of the PROSPECT model to estimate leaf chlorophyll, $$C_{ab}$$, carotenoid, $$C_{c}$$, water, $$C_{w}$$, and dry matter, $$C_{m}$$, contents from leaf reflectance measurements in the context of phenotyping experiments. An algorithmic reflectance and transmittance model for plant tissue. The mesophyll structure parameter, $$N$$, characterizes the number of homogenous elementary layers that constitute the leaf. Besides, the refractive index was also recalibrated. Young, A. and Britton, G. (1990) Carotenoids and stress, in Stress Responses in Plants: Adaptation and Acclimation Mechanisms (Alscher, R.G. energy reflected at each light frequency is named reflectance spectrum, sometimes abbreviated by spectra or by reflectance. 7b, d), the effect of the brown pigments was negligible as expected since they do not absorb in these longer wavelengths. Finally, this study indicates that non-destructive methods may provide similar or better precision of chlorophyllian pigments and water contents as compared to classical destructive measurements [29]. Consequently, poor predictions may be observed when applying them on cases that are not represented in the training dataset, i.e. 1999;68(3):273–80. Computers and Electronics in Agriculture. 2012;110(6):1271–9. Estimates of $$f_{wb}$$ using P4, P4b, P5, and P5b, were higher ($$f_{wb}$$ ≈ 0.07) than for P3, P3b, PD and PDb ($$f_{wb}$$ ≈ 0.03). Reflectance measurements were conducted for each of the six leaves used for destructive measurements. Remote Sens Environ.