Molecular Weight (g/mol) Enter FW or select from the list below. But all cells will not grow. Buffer Calculator is an online tool for buffer pH calculations. Approximate pH values are calculated with Henderson-Hasselbalch equation. If you want to avoid co2 completely you can use L15 media which uses HEPES buffer system. This calculator is valid for a buffer of a weak acid and it's conjugate base of the same system.For example if a system contains both CH 3 COOH and CH 3 COONa then the pH of this buffer can be calculated. Quick order. We use L15 media for MDA-MB231 cells. Weight (gram) Volume (ml) Concentration (M) In this protocol, we will use a solid powder of pure HEPES (MW 238.3 g/mol) to make 100 mL of 0.1 M HEPES, pH 7.4. Note that here [CH 3 COOH] = [CA] and [CH 3 COONa] = [CB].This equation is also known as Henderson-Hasselbalch equation. Many biological experiments require the use of buffers to maintain an effective pH, which is important since proteins and enzymes are sensitive to changes in pH, and choosing the Contact us. But all cells will not grow. Order info . It also provides a list of pKa values of buffers commonly used in biology and biochemistry. Recipes can be automatically calculated for desired volume. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. Instructions and recipes for preparation of commonly used physiological buffers such as PBS and HBSS. HEPES is a buffer that can be used to control the pH of many solutions, and this particular buffer is used in our lab to make assay buffers for fluorogenic substrate assays to measure enzyme activity in the presence of various inhibitory substances. Learn more >> AAT Bioquest. Buffer Calculator; Peptide mapping tool; Useful Links; Instrument logs; SRF; A-Z; This tool has helped you make litres of buffer (target 1,000,000 litres by September 2018) The main purpose of the site is to give access to the software written for the design of thermodynamically corrected buffers. For your convenience, the biological buffers table contain values of pKa 0, d(pKa 0)/dt at 298.25 K, and a calculator that allows you to estimate pKa values of each buffer at temperatures form 3 o C to 37 o C, and concentrations from 1 to 500mM for "white background" buffers and 1 to 130mM for "brown background" buffers. To make a desired concentration made with a fixed mass, enter "Concentration desired" and "Mass available" above Materials.